The principle of supercritical CO2 fluid extraction (SFE) and separation process is to use the relationship between the solubility of supercritical CO2 fluid and its density, that is, to use the change of pressure and temperature to the solubility of supercritical CO2 fluid. In the supercritical state, the supercritical fluid is contacted with the substance to be separated, so that it selectively extracts the polar size and molecular weight components in sequence. The extract obtained corresponding to each pressure range cannot be single, but the conditions can be controlled to obtain the best proportion of mixed components, and then the supercritical CO2 fluid is turned into a gaseous state by decompression and heating in the separation kettle, and the extracted substance is completely or basically precipitated, so as to achieve the purpose of separation and purification. The supercritical CO2 fluid extraction process is a combination of extraction and separation processes.

1. Supercritical CO2 extraction can be extracted at close to room temperature (35~ 40 ° C) and under CO2 gas (the critical temperature of CO2 is 31 ° C, the pressure is 7.38 MPa), effectively preventing the oxidation and escape of heat-sensitive substances. Therefore, the active ingredients of medicinal plants are retained in the extract, and the substances with high boiling point, low volatility, and easy pyrolysis can be extracted at temperatures far below their boiling point.
2. The use of SFE is the cleanest extraction method. Since no organic solvents are used throughout the process, the extract has no residual solvent substances, thus preventing the presence of harmful substances to the human body and environmental pollution during the extraction process, ensuring 100% pure naturalness.
3. To extract substances with large molecular weight and large polarity, it is necessary to selectively inject an entrainer that is similar to the supercritical CO2 fluid to participate in the extraction. The entrainer is used to dissolve the insoluble or slightly soluble substances in the material and the supercritical CO2 fluid. With the supercritical CO2 fluid taken out to the separator for separation, this method requires a subsequent process of desolvation.
4. Extraction and separation are combined into one. When the CO2 fluid of the saturated dissolved substance enters the separator, due to a drop in pressure or a change in temperature, the CO2 and the extract quickly become two phases (gas-liquid separation) and are immediately separated. Not only is the extraction efficiency high, but also the energy consumption is low, the production efficiency is improved and the cost is reduced.
4. CO2 is an inactive gas that does not undergo chemical reactions during extraction and is a non-flammable gas. It is odorless, odorless, non-toxic, and safe.
5. CO2 gas is inexpensive, has high purity, is easy to produce, and can be recycled during production, thus effectively reducing costs.
6. Pressure and temperature can both be used to adjust the parameters of the extraction process. By changing the temperature and pressure to achieve the purpose of extraction, pressure fixation can also separate substances by changing the temperature. On the contrary, by fixing the temperature, the extract can be separated by reducing the pressure, so the process is simple and easy to master, and the extraction speed is fast.

Supercritical CO2 extraction has a wide range of applications. For example, in the pharmaceutical industry, it can be used for the extraction of active ingredients of Chinese herbal medicine, the purification of heat-sensitive biological products, and the separation of lipid mixtures; in the food industry, the extraction of hops, pigment extraction, etc.; in the fragrance industry, the purification of natural and synthetic flavors; in the separation of mixtures in the chemical industry, etc. Specific applications can be divided into the following aspects:
1. Extract non-polar fat-soluble substances from plants.
2. Lipid lipid extraction from different microorganisms, or removal of lipid components from sugars and proteins;
3. Extract anti-cancer substances from a variety of plants, especially taxol from the bark and branches and leaves of Taxus chinensis to prevent and treat cancer;
4. Vitamins, mainly the extraction of fat-soluble vitamins.
5. Purification of various active substances (natural or synthetic), removing unwanted molecules (such as pesticides from vegetable extracts) or "residues" to obtain purified products;
6. Extraction of various natural antibacterial, fragrance or antioxidant extracts.
7. Extraction of natural pigments.
8. Design the corresponding extraction process, and use the corresponding equipment to extract bioactive molecules, alkaloids, phenols, and saponins according to the process.

The selection of equipment should be based on the specific needs of the user, and we will make reasonable recommendations for the user.
1. Features of our company's equipment:
The rationality of the configuration of the supercritical CO2 extraction equipment is very important, which is related to whether the operation of the equipment is simple and easy to learn, whether the operation is stable, and affects the extraction efficiency. According to the provided configuration, it can be analyzed whether the supercritical CO2 is saturated during the extraction process.
(1) Continuous independent extraction, high production efficiency, online "one-click recovery" of gaseous CO2 (small equipment or experimental equipment is not recommended to add CO2 recovery function).
(2) CO2 cylinder pressure can be used to less than 1MPa. (Conventional equipment CO2 cylinder pressure is the lowest, can only be used to 4MPa to 4.5MPa).
(3) The extraction kettle pressure is recovered to less than or equal to 1 MPa emptying (the total installed capacity of the equipment is greater than 58L, and a gaseous CO2 recovery system is recommended).
(4) Optional: Clamp type - extraction kettle clamp type quick opening function. (Large equipment is clamp type)
Threaded - the equipment is cost-effective.
(5) High production efficiency, greatly reducing the cost of production consumables.
(6) The extraction pressure is automatically adjusted, the extraction is fast and stable, and there is a differential pressure protection function (to prevent the occurrence of barrel expansion due to material blockage of the cartridge filter). When the extraction pressure reaches the set pressure value, CO2 is automatically circulated.
(7) The pressure of the three separators is automatically adjusted.
(8) Equipped with an anti-icing device (our company's patented technology).
(9) Condenser, gaseous CO2 enters from one interface, and then exits liquid CO2 from another outlet to the storage tank after going through the disc-shaped condenser. This condensation method has the highest efficiency for gaseous CO2 liquefaction, because we are equipped with an anti-ice blocking device, and there will be no ice blocking during the production process. Therefore, using this form of condensation is the best solution.
(10) Equipment cleaning, together with automatic cleaning of the CO2 circulating main pump.

1. Material slicing and shredding machine.
2. Wall breaker, granulator.
3. Dehydration drying equipment (according to the user's choice, such as room temperature drying, positive temperature drying, vacuum freeze drying).
4. Crusher (usually an ordinary crusher or a frozen crusher).
5. Supercritical CO2 extraction equipment.
6. Concentrator (if an entrainer is involved in the extraction, a rotary evaporator or a thin film evaporator is generally used.)
7. Short-range molecular distillation apparatus.
8. Chromatography column.